Protein_Domain
mSA2

Part:BBa_K1896000:Design

Designed by: Bob Van Hove, Maarten Van Brempt   Group: iGEM16_UGent_Belgium   (2016-10-12)


Monomeric Streptavidin (mSA2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 379
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is compatible with the RFC25 "Freiburg standard", which is used to assemble fusion proteins. Restriction sites for NgoMIV and AgeI have been inserted downstream of the start codon and upstream of the stop codon, respectively.

Source

Combination of streptavidin (Streptomyces avidinii) and rhizavidin (Rhizobium etli), engineered through homology modeling [1].

References

  1. Lim, K. H., Huang, H., Pralle, A., & Park, S. (2013). Stable, high‐affinity streptavidin monomer for protein labeling and monovalent biotin detection. Biotechnology and bioengineering, 110(1), 57-67.